ppgpp (Jena Bioscience)

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Jena Bioscience ppgpp
Ppgpp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppgpp/Guanosine-3'%2C5'-bisdiphosphate/pm39945756-105-8-10
Average 93 stars, based on 11 article reviews

ppgpp - by Bioz Stars, 2026-09

93/100 stars

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In Vitro:

Article Title: Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing.
Article Snippet: In vitro transcription reactions for the Cba ppGpp riboswitch and C69A ppGpp riboswitch variant contained 500nM NusA and, when present, 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience). .. In vitro transcription reactions for the Cba ppGpp riboswitch and C69A ppGpp riboswitch variant contained 500nM NusA and, when present, 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience). ..

Variant Assay:

Derivative Assay:

Article Title: Single-stranded DNA binding to the transcription factor PafBC triggers the mycobacterial DNA damage response.
Article Snippet: Lyophilized ssDNA and ssRNA oligonucleotides (Microsynth) were dissolved in water, the concentration was determined by absorbance at 260 nm, and the ssDNA was frozen and stored at −20°C until further use. .. Nucleotide second messengers including cAMP, cGMP (cyclic GMP), cyclic- di- AMP (cyclic diadenosine monophosphate), cyclic- di- GMP (cyclic diguanosine monophosphate), 2′,3′- cGAMP and 3′,3′- cGAMP, ppGpp (guanosine- 3′,5′- bisdiphosphate), and pppGpp (all derived from Jena Bioscience) were dissolved in water and then frozen and stored at −20°C until further use. .. The in vitro transcription reactions were prepared as 20- μl reactions, which included 20 μM DFHBI- 1T (Jena Bioscience), 1 mM rNTPs (Ribonucleotide Solution Mix, NEB), 0.05 μM DNA template, 0.5 μM RNAP, 2.5 μM SigA- RbpA, 10 μM CarD, 5 μM PafBC, and 20 μM ssDNA/ssRNA/nucleotide second messengers in a reaction buffer [20 mM Hepes- KOH (pH 7.8), 12 mM MgCl2, 50 mM KCl, and 1 mM DTT] supplemented with RiboLock RNase Inhibitor (0.5 U/μl; Thermo Fisher Scientific) and bovine serum album (BSA) (0.05 mg/ml; Thermo Fisher Scientific).

Article Title: Single-stranded DNA binding to the transcription factor PafBC triggers the mycobacterial DNA damage response
Article Snippet: Lyophilized ssDNA and ssRNA oligonucleotides (Microsynth) were dissolved in water, the concentration was determined by absorbance at 260 nm, and the ssDNA was frozen and stored at −20°C until further use. .. Nucleotide second messengers including cAMP, cGMP (cyclic GMP), cyclic-di-AMP (cyclic diadenosine monophosphate), cyclic-di-GMP (cyclic diguanosine monophosphate), 2′,3′-cGAMP and 3′,3′-cGAMP, ppGpp (guanosine-3′,5′-bisdiphosphate), and pppGpp (all derived from Jena Bioscience) were dissolved in water and then frozen and stored at −20°C until further use. .. The in vitro transcription reactions were prepared as 20-μl reactions, which included 20 μM DFHBI-1T (Jena Bioscience), 1 mM rNTPs (Ribonucleotide Solution Mix, NEB), 0.05 μM DNA template, 0.5 μM RNAP, 2.5 μM SigA-RbpA, 10 μM CarD, 5 μM PafBC, and 20 μM ssDNA/ssRNA/nucleotide second messengers in a reaction buffer [20 mM Hepes-KOH (pH 7.8), 12 mM MgCl 2 , 50 mM KCl, and 1 mM DTT] supplemented with RiboLock RNase Inhibitor (0.5 U/μl; Thermo Fisher Scientific) and bovine serum album (BSA) (0.05 mg/ml; Thermo Fisher Scientific).

other:

Article Title: c-di-GMP inhibits rRNA methylation and impairs ribosome assembly in the presence of kanamycin
Article Snippet: ppGpp , Jena bioscience , NU-885S.

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