ppgpp (Jena Bioscience)

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Jena Bioscience ppgpp
Ppgpp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppgpp/Guanosine-3'%2C5'-bisdiphosphate/pm39945756-105-8-10
Average 95 stars, based on 9 article reviews

ppgpp - by Bioz Stars, 2026-10

95/100 stars

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In Vitro:

Article Title: Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing.
Article Snippet: In vitro transcription reactions for the Cba ppGpp riboswitch and C69A ppGpp riboswitch variant contained 500nM NusA and, when present, 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience). .. In vitro transcription reactions for the Cba ppGpp riboswitch and C69A ppGpp riboswitch variant contained 500nM NusA and, when present, 250 μM ppGpp (Guanosine-3’,5’-bisdiphosphate) (Jena Bioscience). .. Translesion DNA synthesis using Sulfolobus DNA polymerase IV (New England Biolabs) was performed by incubating two 100 μl reactions containing the purified PCR product, 1X ThermoPol Buffer, 0.2mM dNTPs, 0.02 U/μl Vent (exo-) DNA Polymerase, and 0.02 U/μl Nature Communications | (2023) 14:7839 14 SulfolobusDNA Polymerase IV at 55 °C for one hour in a thermal cycler with a heated lid set to 105 °C24,71.

Variant Assay:

Derivative Assay:

Article Title: Single-stranded DNA binding to the transcription factor PafBC triggers the mycobacterial DNA damage response.
Article Snippet: Lyophilized ssDNA and ssRNA oligonucleotides (Microsynth) were dissolved in water, the concentration was determined by absorbance at 260 nm, and the ssDNA was frozen and stored at −20°C until further use. .. Nucleotide second messengers including cAMP, cGMP (cyclic GMP), cyclic- di- AMP (cyclic diadenosine monophosphate), cyclic- di- GMP (cyclic diguanosine monophosphate), 2′,3′- cGAMP and 3′,3′- cGAMP, ppGpp (guanosine- 3′,5′- bisdiphosphate), and pppGpp (all derived from Jena Bioscience) were dissolved in water and then frozen and stored at −20°C until further use. .. The in vitro transcription reactions were prepared as 20- μl reactions, which included 20 μM DFHBI- 1T (Jena Bioscience), 1 mM rNTPs (Ribonucleotide Solution Mix, NEB), 0.05 μM DNA template, 0.5 μM RNAP, 2.5 μM SigA- RbpA, 10 μM CarD, 5 μM PafBC, and 20 μM ssDNA/ssRNA/nucleotide second messengers in a reaction buffer [20 mM Hepes- KOH (pH 7.8), 12 mM MgCl2, 50 mM KCl, and 1 mM DTT] supplemented with RiboLock RNase Inhibitor (0.5 U/μl; Thermo Fisher Scientific) and bovine serum album (BSA) (0.05 mg/ml; Thermo Fisher Scientific).

other:

Article Title: c-di-GMP inhibits rRNA methylation and impairs ribosome assembly in the presence of kanamycin
Article Snippet: ppGpp , Jena bioscience , NU-885S.

Article Title: Observation of coordinated RNA folding events by systematic cotranscriptional RNA structure probing
Article Snippet: Translesion DNA synthesis using Sulfolobus DNA polymerase IV (New England Biolabs) was performed by incubating two 100 μl reactions containing the purified PCR product, 1X ThermoPol Buffer, 0.2 mM dNTPs, 0.02 U/μl Vent (exo-) DNA Polymerase, and 0.02 U/μl Sulfolobus DNA Polymerase IV at 55 °C for one hour in a thermal cycler with a heated lid set to 105 °C , .

Article Title: Single-stranded DNA binding to the transcription factor PafBC triggers the mycobacterial DNA damage response
Article Snippet: Lyophilized ssDNA and ssRNA oligonucleotides (Microsynth) were dissolved in water, the concentration was determined by absorbance at 260 nm, and the ssDNA was frozen and stored at −20°C until further use.

Review